Ellagic acid was extracted and isolated from Punica granatum fruit Peels. It was structurally identified using different spectroscopic analyses (1H-NMR, 13C-NMR, IR spectrum and ESIMS). The toxicity of ellagic acid against both of T. absoluta eggs and second instar larvae was assessed. It showed high ovicidal and larvicidal activity. Also, the biochemical impacts of ellagic acid on the second instar larvae of T. absoluta were investigated. The target enzymes activities to be tested were the transaminases (GPT and GOT) in addition to alkaline phosphatase (AlP). Ellagic acid caused high declination of all tested enzymes. This declination explain the high biological dysfunction of the treated larvae.